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KMID : 0545119980080010049
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 1 p.49 ~ p.52
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Identification of Multiple Active Forms in Cellulase-xylanase of Aspergillus sp. 8-17 by Active Staining
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Shin, Pyung Gyun
Ahn, Jun Bae/Kim, Chang Young/Jeong, Won Hwa/Ryu, Jin Chang
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Abstract
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A fungal strain able to produce filter paper activity (FPase) was isolated from soil by testing the ability to hydrolyze using filter paper. The isolated strain was identified as an Aspergilus sp. judging from its morphological and microscopical characteristics. The cellulase-xylanase system of Aspergillus sp. 8-17 was detected in situ after gel electrophoresis in the presence of SDS and showed that each protein pattern had a distinct polypeptide composition. β-1,4-Glucanase, cellobiohydrolase, and xylanase activity profiles differ from protein patterns. The Aspergillus sp. 8-17 hydrolytic enzymes responsible for the hydrolysis of β-glucan, MUC, and xylan have multiple active forms.
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KEYWORD
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